![]() Exploring Network Structure, Dynamics, and Function Using Networkx (Los Alamos National Laboratory, 2008). ![]() Complex heatmaps reveal patterns and correlations in multidimensional genomic data. MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations. The Gene Ontology Resource: 20 years and still GOing strong. GeneTrail 3: advanced high-throughput enrichment analysis. Murine single-cell RNA-seq reveals cell-identity- and tissue-specific trajectories of aging. Single-cell analysis of human pancreas reveals transcriptional signatures of aging and somatic mutation patterns. Controlling the false discovery rate: a practical and powerful approach to multiple testing. On a test of whether one of two random variables is stochastically larger than the other. Scikit-learn: machine Learning in Python. Fast, sensitive and accurate integration of single-cell data with Harmony. UMAP: Uniform Manifold Approximation and Projection for Dimension Reduction. From Louvain to Leiden: guaranteeing well-connected communities. BBKNN: fast batch alignment of single cell transcriptomes. SCANPY: large-scale single-cell gene expression data analysis. Current best practices in single‐cell RNA‐seq analysis: a tutorial. Large-scale low-cost NGS library preparation using a robust Tn5 purification and tagmentation protocol. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects. A survey of human brain transcriptome diversity at the single cell level. Full-length RNA-seq from single cells using Smart-seq2. Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice. Aging of the immune system: focus on inflammation and vaccination. ![]() The extracellular matrix: structure, composition, age-related differences, tools for analysis and applications for tissue engineering. Mouse Aging Cell Atlas analysis reveals global and cell type specific aging signatures revision 1. A systematic RNAi screen identifies a critical role for mitochondria in C. ![]() The aged hematopoietic system promotes hippocampal-dependent cognitive decline. Bone marrow rejuvenation accelerates re-endothelialization and attenuates intimal hyperplasia after vascular injury in aging mice. Effect on lifespan of high yield non-myeloablating transplantation of bone marrow from young to old mice. Exposure to a youthful circulation rejuvenates bone repair through modulation of β-catenin. Young bone marrow transplantation preserves learning and memory in old mice. Caloric restriction reprograms the single-cell transcriptional landscape of Rattus norvegicus aging. Rejuvenation of aged progenitor cells by exposure to a young systemic environment. Gpr158 mediates osteocalcin’s regulation of cognition. β2-microglobulin is a systemic pro-aging factor that impairs cognitive function and neurogenesis. Vascular and neurogenic rejuvenation of the aging mouse brain by young systemic factors. The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Blood-borne revitalization of the aged brain. A single-cell transcriptomic atlas characterizes ageing tissues in the mouse. Ageing hallmarks exhibit organ-specific temporal signatures. Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris. Turning back time with emerging rejuvenation strategies. Together, these data lay the groundwork for a systemic understanding of the interplay between blood-borne factors and cellular integrity. We observed an almost universal loss of gene expression with age that is largely mimicked by parabiosis: aged blood reduces global gene expression, and young blood restores it in select cell types. On the pathway level, young blood invokes new gene sets in addition to reversing established ageing patterns, with the global rescue of genes encoding electron transport chain subunits pinpointing a prominent role of mitochondrial function in parabiosis-mediated rejuvenation. Adipose mesenchymal stromal cells, haematopoietic stem cells and hepatocytes are among those cell types that are especially responsive. Here we performed single-cell RNA sequencing on 20 organs to reveal cell-type-specific responses to young and aged blood in heterochronic parabiosis. Although an increasing number of interventions show promise for rejuvenation 2, their effectiveness on disparate cell types across the body and the molecular pathways susceptible to rejuvenation remain largely unexplored. The ability to slow or reverse biological ageing would have major implications for mitigating disease risk and maintaining vitality 1.
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